Fluorescence Imaging

Fluorescence imaging utilizes light produced by fluorescent species that are introduced into the specimen of interest. SETA BioMedicals has developed a series of proprietary probes and lables that are extremely bright and photostable fluorescent dyes for 2P, in-vivo and conventional imaging applications.

Mouse egg cell stained with Square-635 and Square-460

The group of Prof. Weissleder at the Harvard Medical School has synthesized, characterized and tested three compatible imaging probes based on GLP1R targeting peptides to visualize pancreatic beta cells by intravital microscopy. Of the three conjugates (Alexa-488, Alexa-568 and SeTau-647) presented in their paper the performance of SeTau 647 was by far the best in this series of exendin-4-fluorophore conjugates. They determined that SeTau-647 had the highest signal-to-background ratio and the best in vivo imaging properties (2-photon as well as conventional).

The same group used a CD8 antibody labelled with SeTau-647-maleimide (K9-4148) in an intravital time-lapse microscopy (IVM) experiment [33].

Excitation Light Sources Characteristics Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Medium λ abs
[nm]
ε
[M–1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                               
K35
K35
K35
K35
Toluene 410 10,000 495 60 EtOH 430 13,200 552 0.01
K5-3222
K5-3222
Seta-580-NHS
Seta-580-NHS
NH2 PB 7.4 578 90,000 602 55 BSA, D/P=1 585 609 15
K6-207
K6-207
3-DAB
3-DAB
Toluene 445 9,700 582 43 EtOH 460 8,600 665 22 4.0
K6-208
K6-208
2-DAB
2-DAB
Toluene 464 3,600 551 31 EtOH 480 2,900 670 1.1
K7-1500
K7-1500
Q535
Q535
Toluene 542 15,000
K8-1342
K8-1342
Seta-670-NHS
Seta-670-NHS
NH2 PB 7.4 667 180,000 688 7 0.42 BSA, D/P=1.5 681 180,000 696 27 2.43
K8-1346
K8-1346
Seta-670-Azide
Seta-670-Azide
triple-CC PB 7.4 667 180,000 686 7 PB 7.4 667 180,000 686 7
K8-1350
K8-1350
Square-670-Carboxy
Square-670-Carboxy
PB 7.4 656 676 1.7 0.29 6g/L BSA 673 693 29
K8-1351
K8-1351
Square-660-Carboxy
Square-660-Carboxy
PB 7.4 657 182,000 676 3 0.27 2g/L BSA 679 182,000 696 45 3.56
K8-1355
K8-1355
Square-680-Carboxy
Square-680-Carboxy
PB 7.4 654 138,000 670 1 1g/L BSA 688 168,000 708 62
K8-1357
K8-1357
Square-680-NHS
Square-680-NHS
NH2 MeOH 668 208,000 690 10 CHCl3 683 202,000 701 47
K8-1384
K8-1384
Seta-700-NHS
Seta-700-NHS
NH2 PB 7.4 687 177,000 703 6 IgG D/P=1 705 177,000 715 4
K8-1407
K8-1407
Square-650-pH-NHS
Square-650-pH-NHS
NH2 PB 5.6 653 135,000 671 16 1.17 IgG, D/P=0.8
pH=2.0
662 135,000 677 7 1.52
K8-1440
K8-1440
Square-670
Square-670
pH 7.4 659 677 0.09 1 g/L BSA 683 702 50
K8-1500
K8-1500
Square-655
Square-655
PB 7.4 622 177,000 644 0.09 1 g/L BSA 652 188 672 80
K8-1641
K8-1641
Seta-632-Maleimide
Seta-632-Maleimide
SH PB 7.4 633 270,000 642 5
K8-1642
K8-1642
Seta-632-NHS
Seta-632-NHS
NH2 PB 7.4 632 280,000 641 6 IgG, D/P=1 637 647 18
K8-1663
K8-1663
Seta-633-NHS
Seta-633-NHS
NH2 PB 7.4 633 250,000 644 7 0.25 IgG D/P=1 637 250,000 647 26 1.49
K8-1672
K8-1672
Seta-646-NHS
Seta-646-NHS
NH2 PB 7.4 646 207,000 656 10 0.38 IgG, D/P=1 650 207,000 661 33
K8-1682
K8-1682
Seta-660-di-NHS
Seta-660-di-NHS
NH2 PB 7.4 661 220,000 672 11 0.9 IgG D/P=1 665 220,000 675 49
K8-1696
K8-1696
Seta-633-Azide
Seta-633-Azide
triple-CC PB 7.4 633 250,000 644 7 PB 7.4 633 250,000 644 7
K8-3335
K8-3335
Seta-555-NHS
Seta-555-NHS
NH2 PB 7.4 555 155,000 570 7 IgG, D/P=1 556 571 13
K8-3345
K8-3345
Seta-555-DBCO
Seta-555-DBCO
N3 PB 7.4 555 155,000 570 7
K8-3346
K8-3346
Seta-555-Azide
Seta-555-Azide
triple-CC PB 7.4 555 155,000 570 7
K8-5035
K8-5035
Seta-650-NHS
Seta-650-NHS
NH2 PB 7.4 651 200,000 671 28 IgG, D/P=1 654 673 37
K8-5036
K8-5036
Seta-650-Maleimide
Seta-650-Maleimide
SH PB 7.4 652 200,000 672 28
K8-5045
K8-5045
Seta-650-DBCO
Seta-650-DBCO
N3 PB 7.4 653 200,000 674 28
K8-5046
K8-5046
Seta-650-Azide
Seta-650-Azide
triple-CC PB 7.4 651 200,000 671 28
K8-7045
K8-7045
Seta-750-DBCO
Seta-750-DBCO
N3 PB 7.4 754 234,000 782 21
K8-7046
K8-7046
Seta-750-Azide
Seta-750-Azide
triple-CC PB 7.4 750 200,000 779 22
K8-7055
K8-7055
Seta-780-NHS
Seta-780-NHS
NH2 PB 7.4 777 215,000 807 1.2
K9-4142
K9-4142
SeTau-647-di-NHS
SeTau-647-di-NHS
NH2 PB 7.4 650 200,000 694 65 3.2 IgG, D/P=0.5 649 200,000 691 58 3.2
K9-4145
K9-4145
SeTau-633-Ethyl-Ester
SeTau-633-Ethyl-Ester
CHCl3 634 105,000 683 68
K9-4148
K9-4148
SeTau-647-Maleimide
SeTau-647-Maleimide
SH PB 7.4 648 200,000 692 45 3.2 PB 7.4 648 200,000 692 45 3.2
K9-4149
K9-4149
SeTau-647-NHS
SeTau-647-NHS
NH2 PB 7.4 649 200,000 695 61 3.2 IgG, D/P=1 648 200,000 694 59 3.3
K9-4150
K9-4150
SeTau-647
SeTau-647
PB 7.4 647 211,000 693 59 3.1 PB 7.4 647 211,000 693 59 3.1
K8-3402
K8-3402
Zy3-NHS
Zy3-NHS
NH2 PB 7.4 549 150,000 564 4 0.3
K8-3403
K8-3403
Zy3-Maleimide
Zy3-Maleimide
SH PB 7.4 549 150,000 564 4 0.3
K8-1388
K8-1388
Seta-700-di-NHS
Seta-700-di-NHS
NH2 PB 7.4 688 180,000 704 11 IgG D/P=1 696 713 23
K8-1341
K8-1341
Seta-670-Maleimide
Seta-670-Maleimide
SH PB 7.4 667 180,000 688 7
K8-1402
K8-1402
Square-650
Square-650
CHCl3 654 210,000 671 53
K8-1671
K8-1671
Seta-646-Maleimide
Seta-646-Maleimide
SH PB 7.4 647 210,000 657 8 IgG, D/P=1 650 661 31
K8-3010
K8-3010 new
Square-460
Square-460
PB 7.4 467 50,000 515 0.9 6g/L BSA 469 512 8.6
K4-205
K4-205 new
Seta-375-NHS
Seta-375-NHS
NH2 PB 7.4 372 24,500 476 86 3.1 IgG, D/P=5 372 24,500 480 42 3.5
K7-548
K7-548
SeTau-405-Maleimide
SeTau-405-Maleimide
SH PB 7.4 405 13,800 518 51 9.1 EtOH 391 15,000 498 32 8.5
cl-APC
cl-APC
Crosslinked APC
Crosslinked APC
PB 7.4 650 700,000 662
K8-1405
K8-1405
Square-650-pH-Carboxy
Square-650-pH-Carboxy
NH2 PB 5.6 653 135,000 671 16 1.17 pH 9.0 535 48,000 663 9 0.53
K10-6801
K10-6801 new
Seta-PerCP-680
Seta-PerCP-680
PB 7.4 481 380,000 676
K10-7801
K10-7801 new
Seta-APC-780
Seta-APC-780
PB 7.4 651 1,100,000 780
K10-6701
K10-6701 new
Seta-R-PE-670
Seta-R-PE-670
PB 7.4 488 1,100,000 671
K8-7522
K8-7522
Seta-750-NHS
Seta-750-NHS
NH2 PB 7.4 753 230,000 780 14 IgG D/P=1 756 783 17
K10-0775
K10-0775 new
Seta-R-PE-775
Seta-R-PE-775
PB 7.4 488 1,100,000 774
K10-7051
K10-7051 new
Seta-APC-705
Seta-APC-705
PB 7.4 655 1,100,000 703
K4-225
K4-225 new
Seta-385-NHS
Seta-385-NHS
NH2 PB 7.4 384 25,000 512 78 IgG, D/P=6 387 25,000 505 29
K6-5052
K6-5052 new
Seta-405 NHS
Seta-405 NHS
NH2 PB 7.4 401 30,000 452 70 IgG D/P=1 405 453 56
K4-215
K4-215 new
Seta-400-NHS
Seta-400-NHS
NH2 PB 7.4 399 24,000 576 34 IgG D/P=1 401 569 19
K9-4179
K9-4179 new
SeTau-680-NHS
SeTau-680-NHS
NH2 PB 7.4 683 295,000 705 58 2.9 IgG D/P=1 682 703 47 3.1
K9-4154
K9-4154 new
SeTau-647-DBCO
SeTau-647-DBCO
N3 PB 7.4 649 200,000 690 60 3.2
K9-3152
K9-3152 new
SeTau-488-NHS
SeTau-488-NHS
NH2 PBS 7.4 486 59,000 532 27

See also NIR Fluorescence Imaging and Fluorescence Lifetime Imaging.

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