pH Sensing Applications

			Characteristics						Characteristics
Product Number (Specs Sheet)	Product Name (Product Info)	Target Group	Medium	λ abs [nm]	ε [M –1 cm–1]	λ em [nm]	QY [%]	FLT [ns]	Medium	λ abs [nm]	ε [M–1 cm–1]	λ em [nm]	QY [%]	FLT [ns]	Buy
K8-1367 K8-1367	Seta-670-pH-di-NHS Seta-670-pH-di-NHS	NH2	PB 6.0	672	93,000	694	19		IgG, D/P=1	684		703	7
K8-1407 K8-1407	Square-650-pH-NHS Square-650-pH-NHS	NH2	PB 5.6	653	135,000	671	16	1.17	IgG, D/P=0.8 pH=2.0	662	135,000	677	7	1.52
K8-1667 K8-1667	Seta-635-pH-di-NHS Seta-635-pH-di-NHS	NH2	PB 7.4	640	188,000	656	33	1.8	IgG, D/P=1	646		663	21
K8-1405 K8-1405	Square-650-pH-Carboxy Square-650-pH-Carboxy	NH2	PB 5.6	653	135,000	671	16	1.17	pH 9.0	535	48,000	663	9	0.53
K8-1610 K8-1610	Square-635-Carboxy Square-635-Carboxy		pH 7.4	634	180,000	646	9		pH 12.0	520		-	-	-
K8-1612 K8-1612	Square-635-NHS Square-635-NHS	NH2	pH 7.4	634	180,000	646	9		BSA, D/P=1.0	649		663	29
K8-1665 K8-1665	Seta-635-pH-di-Carboxy Seta-635-pH-di-Carboxy		PB 7.4	640	188,000	656	33	1.8	pH 12.0	519		-	-	-
K8-1365 K8-1365	Seta-670-pH-di-Carboxy Seta-670-pH-di-Carboxy		PB 6.0	672	93,000	694	19		pH 11.5	535		-	-	-
K8-1613 K8-1613	Square-635-Maleimide Square-635-Maleimide	SH	pH 7.4	636	180,000	649	8

pH-measurement in cells: Probes such as Square-650-pH-Carboxy and labels such as Square-650-pH-NHS exhibit pKa's in the physiologically relevant pH range and are therefore useful to measure the intercellular pH changes in cells. For more information about these dyes we refer you to the specs sheet of Square-650-pH-Carboxy and Square-650-pH-NHS and the published literature.

pH-probes with alkaline pKa's: The pH-sensitive dyes with pKa's in the alkaline pH range (see table below) are available in probe and reactive formats. Their pKa's range from 8.8 to 10.7.

 

 

Product Number (Specs Sheet)	Product Name (Product Info)	Availability	Protonated form			Deprotonated form	pKa	pH range
			λ abs [nm]	λ em [nm]	QY [%]	λ abs (λ em) [nm]
K8-1365	Seta-670-pH-di-Carboxy	~ 2 weeks	672	694	19	535	8.8 9.6	7.6–11.4
K8-1367	Seta-670-pH-di-NHS	~ 2 weeks
IgG conjugate, D/P=1	–	–	684	703	7	–	–	–
K8-1405	Square-650-pH-Carboxy	~ 2 weeks	653	671	16	535 (663)	7.1	5.3-9.0
K8-1407	Square-650-pH-NHS	in stock
IgG conjugate, D/P=1	–	–	662	677	7	546 (663)	6.3	4.0–8.0
K8-1610	Square-635-Carboxy	in stock	634	646	9	520	10.7	9.6–11.7
K8-1612	Square-635-NHS	in stock
K8-1613	Square-635-Maleimide	in stock	636	649	8
BSA conjugate, D/P=1	–	–	649	663	29	–	–	–
K8-1665	Seta-635-pH-di-Carboxy	~ 2 weeks	640	656	33	519	10.2	9.2-11.8
K8-1667	Seta-635-pH-di-NHS	~ 2 weeks
IgG conjugate, D/P=1	–	–	646	663	21	–	–	–

 

Comparison of the dyes Seta-646-NHS, Seta-670-pH-di-NHS and Seta-635-pH-di-NHS
in a range of universal buffered solutions (pH 5.28 – 12.69)