Product Name: SeTau-647-DBCO
Product Number:
K9-4154 Target Group:
N3Excitation Light Sources:
Medium 1:
PB 7.4λAb max. [nm]:
649ε [M–1 cm–1]:
200000λEm max. [nm]:
690QY [%]:
60FLT [ns]:
3.2Specs Sheet:
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Description
SeTau 647-DBCO (K9-4154) is an extremely bright, hydrophilic click chemistry reagent containing one dibenzylcyclooctyne (DBCO) group for strain-mediated cycloaddition reactions with azides. It has the same excitation wavelength as Cy5, Alexa Fluor™ 647 and can therefore be used with these filter sets. Its emission is at 690 nm.
Applications
Strain-mediated click chemistry reactions with azide-modified reagents and biomolecules.
Advantages
- Suited for excitation with the 647 nm Kr-laser or 650 nm diode laser
- Sensitive; high extinction coefficient and high quantum yield (about twice as high as Alexa 647)
- Well-suited for confocal and 2-photon imaging and fluorescence polarization measurements
- High 2PACS
- pH-insensitive between pH 3 and pH 10
- Good aqueous solubility
- Extremely bright label: SeTau 647: (Φ = 0.60, ε = 200,000 and Φ. ε =120,000) vs Alexa 647: (Φ = 0.33, ε = 270,000 and Φ. ε = 89,100)
- Very high photostability; e.g. compared to fluorescein, Cy5 or Alexa 647
