Seta-780-NHS (K8-7055) is a hydrophilic, amine-reactive label containing one NHS-ester group with almost identical absorption and emission as ICG and can therefore be used with these filter sets.
Applications
Covalent labeling of proteins, amino-modified DNA, and amino-modified oligonucleotides
Fluorescence intensity and fluorescence polarization-based applications
Resonance Energy Transfer (RET)
Flow Cytometry
Immunofluorescence
Gene Expression
Homogeneous Assays
Microarrays
Advantages
Perfectly suited for excitation with the 700-nm, 750-nm, or 780-nm LEDs and diode lasers
Sensitive; high extinction coefficients and the quantum yield in water is as much as twice higher compared to Indocyanine green (ICG)
pH-insensitive between pH 3 and pH 10
Good aqueous solubility; this label does not alter the solubility of bioconjugates
High photostability
Low molecular weight — Seta-780 does not add substantial mass to the conjugates
Ideal for non-radioactive labeling of proteins, amino-modified oligonucleotides, and amino-modified lipids