Links to Selected Products
- Squaraine Rotaxanes
- Labels
- Probes
- Proteins & Conjugates
- Dyes for Imaging
- Dyes for Click Chemistry
- Dyes for Single Molecule Applications
- Dyes for Immunofluorescence
- Dyes for Proteomics
- Dyes for Flow Cytometry
- Dyes for Fluorescence Polarization Assays
- Dyes for FRET Based Assays
- More Products
- More Applications
Applications
- Fluorescence Imaging
- Flow Cytometry
- DNA and Protein Microarrays
- 2-Photon Microscopy
- Superresolution Microscopy
- Single Molecule Applications
- Immunofluorescence
- Click Chemistry
- Fluorescence Lifetime (FLT)
- Fluorescence Polarization
- FRET
- Genomics
- FCS
- pH Sensing Applications
- Plasmonics
- Proteomics
- Albumin Determination
- Cholesterol & Triglycerides Measurement
- Viscosity Measurements
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Urbana, IL, Sept 2023SETA BioMedicals is currently collaborating with the Micron School of Materials Science & Engineering at Boise State University on phase 3 of a DOE funded project to develop new fluorescent dyes for DNA-controlled dye aggregation as a path to create quantum entanglement.
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Urbana, IL, Sept 2021SETA BioMedicals and the Micron School of Materials Science & Engineering at Boise State University are currently collaborating on a DOE funded project.
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Urbana, IL, June 2019SETA BioMedicals will exhibit at the MAF meeting in La Jolla, CA, USA on Aug 20 - 24, 2019! Please visit us at booth 9 to get information about our latest products.
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SETA BioMedicals offers free consultation on the use of SETA products for your application.
Please call us at 217 417 2160.
Welcome to SETA BioMedicals
SETA BioMedicals is the exclusive producer of Squaraine rotaxanes, the world's brightest, chemically-robust and photostable dyes for biomedical applications.
SETA BioMedicals also produces, commercializes, and licenses other proprietary fluorescent detection reagents (fluorescent dyes, probes, labels, tandems, and standards) for biomedical research applications.
These detection reagents can be ordered over the phone at (217) 417 2160, via e-mail at orders@setabiomedicals.com, or with our online shopping cart and are delivered the next day in the continental USA and within days at any other destination around the world.
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Squaraine Rotaxanes - Simply the Best!
Extremely photostable dyes, probes, and labels based on squaraine rotaxanes. These unique fluorescent reporters combine all desirable features of common fluorescent reagents, including high chemical and photochemical stability. Try them yourself - you will not regret it!
Squaraine rotaxane model structure and products
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Click Chemistry Reagents
Azides and proprietary DBCO derivatives of long-lifetime and 2P-imaging dyes with 2PACS of several thousand GM for Cu-catalyzed and Cu-free click chemistry reactions
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Phycobiliprotein Tandem Conjugates
Bright and photostable tandem-conjugates of APC, PE and PerCP with Seta dyes for flow cytometry and immuno-fluorescence applications.
Flow cytometry measurment of blood cells stained with CD3 – Seta-APC-780
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Labeling Technologies
Next generation labels for proteins, oligonucleotides and other biomolecules with exceptional brightness and photostability.
Mouse egg cell stained with
Square-655 and Square-460Mouse egg cell stained with
Square-670-Carboxy -
Lifetime and Intensity-Based Calibration Standards
Fluorescence standards that are utilized in the calibration and validation of fluorescence instruments and luminescence readers as fluorescence reference materials. The standard kits offered by SETA BioMedicals are ready and easy to use!
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Fluorescence Lifetime Labels
Fluorescence lifetime labels with lifetimes from 0.5 to 32 ns for fluorescence lifetime and polarization applications.
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Viscosity-Sensitive Probes and Labels
Large Stokes' shift fluorescent molecular rotors with high sensitivity and a large dynamic range in lifetime and intensity mode
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Exeptional Dyes for 2-Photon Microscopy
Fluorescent probes and labels with exceptionally high 2-photon cross sections close to 10,000 GM based on squaraines and squaraine rotaxanes.
2-PCS comparison of red-emitting Seta and SeTau dyes
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DNA and Protein Microarrays
Our highly fluorescent squaraine rotaxanes exhibit high chemical stability in particular against oxidative reagents such as peroxides or ozone and therefore are excellent detection reagents for use in DNA and protein microarrays where the fuorescence signals of cyanine dyes such as Cy5 and Alexa 647 are strongly dependent on the concentration of ozone during post-hybridization array washing see Microarray Signal Stability Study Test and below.
Comparison of an Alexa-647 and a SeTau-647 rotaxane dye in a study test with labeled avidin at time = 0 and after 2 hours for Alexa 647 and 6 hours for SeTau-647.
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Fluorescence Imaging
Below is a comparison of our Seta-555 and SeTau-647 dyes with other dyes commonly used in such microarrays. These dyes are ideal for both, one- and two-color microarray measurements and they have much higher ozone stability as compared to the dyes of our competitors. Try and experience the difference yourself!
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Single Molecule Applications
In a comparative study of 13 dyes in Nature Chem. Biol., SeTau-647 (K9-4149 and K9-4148), a squaraine rotaxane dye, exhibited the best photobleaching performance in a single molecule application with an exponential lifetime of 105.8 ns (twice as long as the 2nd best dye Atto-647N).
Single molecule study of 13 fluorescent imaging dyes
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Superresolution Microscopy
Seta and SeTau dyes are a class of extremely bright and photostable dyes for fluorescence imaging and are well suited for STED and STORM applications.
STED image of HeLa-M cell nuclei labeled with primary mAB414 antibody and a secondary antibody labeled with SeTau-647.
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2-Photon Microscopy
Compared to conventional cyanine dyes, squaraine and squaraine-rotaxanes exhibit 10 – 50-times higher 2-photon cross-sections in the order of several thousand GM.
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Z-projection of a neuron filled with a squaraine rotaxane-labeled peptide
