Welcome to SETA BioMedicals

SETA BioMedicals is the exclusive producer of Squaraine rotaxanes, the world's brightest, chemically-robust and photostable dyes for biomedical applications.

SETA BioMedicals also produces, commercializes, and licenses other proprietary fluorescent detection reagents (fluorescent dyes, probes, labels, tandems, and standards) for biomedical research applications.

These detection reagents can be ordered over the phone at (217) 417 2160, via e-mail at orders@setabiomedicals.com, or with our online shopping cart and are delivered the next day in the continental USA and within days at any other destination around the world.

TECHNOLOGY HIGHLIGHTS

Squaraine Rotaxanes - Simply the Best!
Extremely stable dyes, probes, labels, tandem conjugates, and fluorescent standards based on squaraine rotaxanes:

These innovative reporters combine all the desirable properties of conventional fluorophores, in addition to exeptional chemical an photochemical stability and high brightness.

They are available as imaging probes and in reactive form with NHS-ester, maleimide, azide, and DBCO modification for a wide range of applications.

Experience the performance of squaraine rotaxane dyes firsthand - we guarantee that you will not be disappointed!
Click Chemistry Reagents
Azides and proprietary DBCO derivatives of long-lifetime and 2P-imaging dyes with 2PACS of several thousand GM for Cu-catalyzed and Cu-free click chemistry reactions
Phycobiliprotein Tandem Conjugates
Bright and photostable tandem-conjugates of APC, PE, and PerCP with Seta dyes for flow cytometry and immuno-fluorescence applications.

Blood cells stained with CD3-Seta-APC-780
Labeling Technologies
Next generation labels for proteins, oligonucleotides, and other biomolecules with exceptional brightness and photostability.

Mouse egg cell stained with
Square-655 and Square-460 Mouse egg cell stained with
Square-670-Carboxy
Lifetime and Intensity-Based Calibration Standards
Fluorescence standards that are utilized in the calibration and validation of fluorescence instruments and luminescence readers as fluorescence reference materials. The standard kits offered by SETA BioMedicals are ready and easy to use!
Fluorescence Lifetime Labels
Fluorescence lifetime labels with lifetimes from 0.5 to 32 ns for fluorescence lifetime and polarization applications.

Fluorescence Lifetime Assay
Viscosity-Sensitive Probes and Labels
Large Stokes' shift fluorescent molecular rotors with high sensitivity and a large dynamic range in lifetime and intensity mode
Exeptional Dyes for 2-Photon Microscopy
Fluorescent probes and labels with exceptionally high 2-photon cross sections close to 10,000 GM based on squaraines and squaraine rotaxanes.

2-PCS comparison of red-emitting Seta and SeTau dyes

APPLICATIONS

DNA and Protein Microarrays

Our highly fluorescent squaraine rotaxanes exhibit excellent chemical stability in particular against oxidative reagents e.g. peroxides or ozone and therefore are excellent detection reagents for use in DNA and protein microarrays. Nevertheless, our customers found that the fluorescence signals of cyanine dyes such as Cy5, Atto and Alexa dyes are strongly dependent on the concentration of ozone during array washing - see our Microarray Signal Stability Comparison and below.

Images of the microarray scans of a SeTau-647 rotaxane dye-labeled-neutravidin bound to an immobilized, biotinylated antibody at time = 0 and after 6 hours (left) and an Alexa-647-labeled neutravidin at time = 0 and after 2 hours (right).
Microarray Imaging

Below is a comparison of our Seta-555 and SeTau-647 dyes with other dyes commonly used in such microarrays. These dyes are ideal for both, one- and two-color microarray measurements and they have much higher ozone stability as compared to other dyes. Try and experience the difference yourself!
Single Molecule Applications

In a comparative study of 13 dyes in Nature Chem. Biol., SeTau-647 (K9-4149 and K9-4148), a squaraine rotaxane dye, exhibited the best photobleaching performance in a single molecule application with an exponential lifetime of 105.8 ns (twice as long as the 2^nd best dye Atto-647N).

Single molecule study of 13 fluorescent imaging dyes
Superresolution Microscopy

Seta and SeTau dyes are a class of extremely bright and photostable dyes for fluorescence imaging and are well suited for STED and STORM applications.

STED image of HeLa-M cell nuclei labeled with primary mAB414 antibody and a secondary antibody labeled with SeTau-647.
2-Photon Microscopy

Compared to conventional cyanine dyes, squaraine and squaraine-rotaxanes exhibit 10 – 50-times higher 2-photon cross-sections in the order of several thousand GM.

Z-projection of a neuron filled with a squaraine rotaxane-labeled peptide


Mouse egg cell stained with Square-655 and Square-460	Mouse egg cell stained with Square-670-Carboxy